Peptide Library Design Strategies for Epitope Mapping

Overlapping libraries (e.g. 15-mers with 10-aa offset) map linear B-cell epitopes.

Truncation libraries identify the minimal active sequence.

Alanine scanning pinpoints key residues for binding and SAR.

Positional scanning systematically explores each position.

Random libraries substitute chosen positions with all 20 amino acids.

Specs: 5-25 aa, 1-100 mg/peptide, 96-well plate delivery, minimum 48 peptides, parallel capacity up to 1,500 peptides.